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t 84 cells  (ATCC)


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    Structured Review

    ATCC t 84 cells
    T 84 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t+84+cells/T84/pmc12510010-241-8-13
    Average 96 stars, based on 1520 article reviews
    t 84 cells - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Bacteria:

    Article Title: Immunogenicity and preclinical efficacy characterization of ShecVax, a combined vaccine against Shigella and enterotoxigenic Escherichia coli .
    Article Snippet: .. Adherent bacteria (CFUs; in %) were collected, cultured, counted, and referred to the CFUs of bacteria treated with the control sera (100%). (C) Serum antibody neutralization against ETEC STa enterotoxicity using T-84 cells (ATCC) and an EIA cyclic GMP kit (Enzo Life Sciences). (D) Serum antibody neutralization against ETEC LT enterotoxicity using T-84 cells and an EIA cAMP kit. ..

    Cell Culture:

    Article Title: Immunogenicity and preclinical efficacy characterization of ShecVax, a combined vaccine against Shigella and enterotoxigenic Escherichia coli .
    Article Snippet: .. Adherent bacteria (CFUs; in %) were collected, cultured, counted, and referred to the CFUs of bacteria treated with the control sera (100%). (C) Serum antibody neutralization against ETEC STa enterotoxicity using T-84 cells (ATCC) and an EIA cyclic GMP kit (Enzo Life Sciences). (D) Serum antibody neutralization against ETEC LT enterotoxicity using T-84 cells and an EIA cAMP kit. ..

    Article Title: Catalytic region mimetics in Na+/H+ exchanger regulatory factor 4 suppress guanylate cyclase 2C activity to regulate enterotoxin triggered diarrhea
    Article Snippet: Cells were transfected with human HA-GCC (HA tag was inserted at amino-acid position 39) cDNA by using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer's protocol. .. The transfected cells were studied after 48 h. T-84 cells were obtained from ATCC and cultured in DMEM/F-12 10% FBS, 1% penicillin-streptomycin. .. Cells were transfected with the ratiometric FRET senor Cygnet 2.1 ( ) using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer's protocol.

    Control:

    Article Title: Immunogenicity and preclinical efficacy characterization of ShecVax, a combined vaccine against Shigella and enterotoxigenic Escherichia coli .
    Article Snippet: .. Adherent bacteria (CFUs; in %) were collected, cultured, counted, and referred to the CFUs of bacteria treated with the control sera (100%). (C) Serum antibody neutralization against ETEC STa enterotoxicity using T-84 cells (ATCC) and an EIA cyclic GMP kit (Enzo Life Sciences). (D) Serum antibody neutralization against ETEC LT enterotoxicity using T-84 cells and an EIA cAMP kit. ..

    Neutralization:

    Article Title: Immunogenicity and preclinical efficacy characterization of ShecVax, a combined vaccine against Shigella and enterotoxigenic Escherichia coli .
    Article Snippet: .. Adherent bacteria (CFUs; in %) were collected, cultured, counted, and referred to the CFUs of bacteria treated with the control sera (100%). (C) Serum antibody neutralization against ETEC STa enterotoxicity using T-84 cells (ATCC) and an EIA cyclic GMP kit (Enzo Life Sciences). (D) Serum antibody neutralization against ETEC LT enterotoxicity using T-84 cells and an EIA cAMP kit. ..

    Article Title: Immunogenicity and preclinical efficacy characterization of ShecVax, a combined vaccine against Shigella and enterotoxigenic Escherichia coli .
    Article Snippet: .. To examine antibody neutralization activities against ETEC STa and LT enterotoxicity, STa, CT (LT homolog), T-84 cells (CCL-248; ATCC, Manassas, Virginia, USA), and a cyclic AMP kit or a cGMP EIA kit (Enzo Life Sciences, Farmingdale, NY, USA) were used. ..

    Article Title: Immunogenicity and preclinical efficacy characterization of ShecVax, a combined vaccine against Shigella and enterotoxigenic Escherichia coli
    Article Snippet: .. To examine antibody neutralization activities against ETEC STa and LT enterotoxicity, STa, CT (LT homolog), T-84 cells (CCL-248; ATCC, Manassas, Virginia, USA), and a cyclic AMP kit or a cGMP EIA kit (Enzo Life Sciences, Farmingdale, NY, USA) were used. ..

    Article Title: MecVax supplemented with CFA MEFA-II induces functional antibodies against 12 adhesins (CFA/I, CS1–CS7, CS12, CS14, CS17, and CS21) and 2 toxins (STa, LT) of enterotoxigenic Escherichia coli (ETEC)
    Article Snippet: .. Sera from mice in each treatment group were examined for neutralization activities against STa and CT enterotoxicity using T-84 cells (CCL-248, ATCC) and a cyclic GMP or AMP EIA kit (Enzo Life Sciences, Farmingdale, NY, USA). .. As we described previously ( , , , ), 30 μL mouse serum sample pooled from each group was mixed with 2 ng STa toxin (BEI Resources, ATCC) or 30 ng CT (Sigma; we increased CT from 10 ng to 30 ng in an attempt to differentiate neutralization activity against CT from antibodies from the vaccine antigen versus dmLT adjuvant) and incubated at room temperature for 30 min and then transferred to a well of a 24-well tissue culture plate (Falcon) containing 95%–100% confluent monolayered T-84 cells and incubated in a CO 2 incubator for 1 h (for cGMP) or 3 h (for cAMP).

    Article Title: Enterotoxigenic E. coli vaccine candidate MecVax with protein antigens prepared from animal-free media is equally immunogenic and protective against adhesins CFA/I, CS1 to CS6 and toxins LT and STa
    Article Snippet: .. Mouse sera were also examined for antibody neutralization against STa and LT (CT homolog) enterotoxicity by using T-84 cells (CCL-248; ATCC) and a cyclic AMP or GMP EIA kit (Enzo Life Science; Farmingdale, NY). .. As described previously ( 17 , 26 ), 30 μl heat-inactivated sera pooled from each group was mixed with 2 ng STa (BEI Resources Repository) or 10 ng CT (Sigma), after incubation at room temperature for 30 min, each toxin/serum mixture was added to confluent T-84 cells, with CT/serum mixture incubated for 1 h (STa/serum, for cGMP) or 3 h (CT/serum, for cAMP) in a CO 2 incubator.

    Article Title: MecVax supplemented with CFA MEFA-II induces functional antibodies against 12 adhesins (CFA/I, CS1-CS7, CS12, CS14, CS17, and CS21) and 2 toxins (STa, LT) of enterotoxigenic Escherichia coli (ETEC).
    Article Snippet: .. Mouse serum antibody neutralization against STa and CT enterotoxicity Sera from mice in each treatment group were examined for neutralization activities against STa and CT enterotoxicity using T-84 cells (CCL-248, ATCC) and a cyclic GMP or AMP EIA kit (Enzo Life Sciences, Farmingdale, NY, USA). .. As we described previously (24, 25, 28, 29), 30 μL mouse serum sample pooled from each group was mixed with 2 ng STa toxin (BEI Resources, ATCC) or 30 ng CT (Sigma; we increased CT from 10 ng to 30 ng in an attempt to differentiate neutralization activity against CT from antibodies from the vaccine antigen versus dmLT adjuvant) and incubated at room temperature for 30 min and then transferred to a well of a 24-well tissue culture plate (Falcon) containing 95%– 100% confluent monolayered T-84 cells and incubated in a CO2 incubator for 1 h (for cGMP) or 3 h (for cAMP).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Immunogenicity and preclinical efficacy characterization of ShecVax, a combined vaccine against Shigella and enterotoxigenic Escherichia coli .
    Article Snippet: .. Adherent bacteria (CFUs; in %) were collected, cultured, counted, and referred to the CFUs of bacteria treated with the control sera (100%). (C) Serum antibody neutralization against ETEC STa enterotoxicity using T-84 cells (ATCC) and an EIA cyclic GMP kit (Enzo Life Sciences). (D) Serum antibody neutralization against ETEC LT enterotoxicity using T-84 cells and an EIA cAMP kit. ..

    Article Title: Immunogenicity and preclinical efficacy characterization of ShecVax, a combined vaccine against Shigella and enterotoxigenic Escherichia coli .
    Article Snippet: .. To examine antibody neutralization activities against ETEC STa and LT enterotoxicity, STa, CT (LT homolog), T-84 cells (CCL-248; ATCC, Manassas, Virginia, USA), and a cyclic AMP kit or a cGMP EIA kit (Enzo Life Sciences, Farmingdale, NY, USA) were used. ..

    Article Title: Immunogenicity and preclinical efficacy characterization of ShecVax, a combined vaccine against Shigella and enterotoxigenic Escherichia coli
    Article Snippet: .. To examine antibody neutralization activities against ETEC STa and LT enterotoxicity, STa, CT (LT homolog), T-84 cells (CCL-248; ATCC, Manassas, Virginia, USA), and a cyclic AMP kit or a cGMP EIA kit (Enzo Life Sciences, Farmingdale, NY, USA) were used. ..

    Article Title: MecVax supplemented with CFA MEFA-II induces functional antibodies against 12 adhesins (CFA/I, CS1–CS7, CS12, CS14, CS17, and CS21) and 2 toxins (STa, LT) of enterotoxigenic Escherichia coli (ETEC)
    Article Snippet: .. Sera from mice in each treatment group were examined for neutralization activities against STa and CT enterotoxicity using T-84 cells (CCL-248, ATCC) and a cyclic GMP or AMP EIA kit (Enzo Life Sciences, Farmingdale, NY, USA). .. As we described previously ( , , , ), 30 μL mouse serum sample pooled from each group was mixed with 2 ng STa toxin (BEI Resources, ATCC) or 30 ng CT (Sigma; we increased CT from 10 ng to 30 ng in an attempt to differentiate neutralization activity against CT from antibodies from the vaccine antigen versus dmLT adjuvant) and incubated at room temperature for 30 min and then transferred to a well of a 24-well tissue culture plate (Falcon) containing 95%–100% confluent monolayered T-84 cells and incubated in a CO 2 incubator for 1 h (for cGMP) or 3 h (for cAMP).

    Article Title: Enterotoxigenic E. coli vaccine candidate MecVax with protein antigens prepared from animal-free media is equally immunogenic and protective against adhesins CFA/I, CS1 to CS6 and toxins LT and STa
    Article Snippet: .. Mouse sera were also examined for antibody neutralization against STa and LT (CT homolog) enterotoxicity by using T-84 cells (CCL-248; ATCC) and a cyclic AMP or GMP EIA kit (Enzo Life Science; Farmingdale, NY). .. As described previously ( 17 , 26 ), 30 μl heat-inactivated sera pooled from each group was mixed with 2 ng STa (BEI Resources Repository) or 10 ng CT (Sigma), after incubation at room temperature for 30 min, each toxin/serum mixture was added to confluent T-84 cells, with CT/serum mixture incubated for 1 h (STa/serum, for cGMP) or 3 h (CT/serum, for cAMP) in a CO 2 incubator.

    Article Title: MecVax supplemented with CFA MEFA-II induces functional antibodies against 12 adhesins (CFA/I, CS1-CS7, CS12, CS14, CS17, and CS21) and 2 toxins (STa, LT) of enterotoxigenic Escherichia coli (ETEC).
    Article Snippet: .. Mouse serum antibody neutralization against STa and CT enterotoxicity Sera from mice in each treatment group were examined for neutralization activities against STa and CT enterotoxicity using T-84 cells (CCL-248, ATCC) and a cyclic GMP or AMP EIA kit (Enzo Life Sciences, Farmingdale, NY, USA). .. As we described previously (24, 25, 28, 29), 30 μL mouse serum sample pooled from each group was mixed with 2 ng STa toxin (BEI Resources, ATCC) or 30 ng CT (Sigma; we increased CT from 10 ng to 30 ng in an attempt to differentiate neutralization activity against CT from antibodies from the vaccine antigen versus dmLT adjuvant) and incubated at room temperature for 30 min and then transferred to a well of a 24-well tissue culture plate (Falcon) containing 95%– 100% confluent monolayered T-84 cells and incubated in a CO2 incubator for 1 h (for cGMP) or 3 h (for cAMP).

    Transfection:

    Article Title: Catalytic region mimetics in Na+/H+ exchanger regulatory factor 4 suppress guanylate cyclase 2C activity to regulate enterotoxin triggered diarrhea
    Article Snippet: Cells were transfected with human HA-GCC (HA tag was inserted at amino-acid position 39) cDNA by using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer's protocol. .. The transfected cells were studied after 48 h. T-84 cells were obtained from ATCC and cultured in DMEM/F-12 10% FBS, 1% penicillin-streptomycin. .. Cells were transfected with the ratiometric FRET senor Cygnet 2.1 ( ) using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer's protocol.



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